Bioconjugation Research Associate

Who We Are Bolt Biotherapeutics, based in the San Francisco Bay Area, is a clinical-stage biotechnology company developing Boltbody™ Immune-stimulating Antibody Conjugates (ISACs), a new class of immuno-oncology therapeutics. This is a unique opportunity to join and build, with like-minded colleagues, a company that will transform the lives of individuals with cancer.

At Bolt Biotherapeutics, we are seeking for highly motivated Research Associate to join our research bioconjugation team. The primary responsibility of the successful candidate is to perform variety of antibody conjugation chemistries and techniques to support discovery and pipeline programs.

Responsibilities:

• Perform conjugation (lysine, cysteine, site specific etc.) of antibodies to diverse linker-payloads on mg to gram scale, following established protocols and procedures.
• Conduct purification of the antibody drug conjugates utilizing various chromatographic techniques (affinity chromatography, size exclusion chromatography, ion exchange chromatography, membrane filtration (TFF) etc.)
• Perform analytical characterization of the antibodies and bioconjugates using analytical techniques including UV-Vis, HIC, SEC, RP-HPLC, and LC-MS.
• Maintain accurate records of experiments, results and analytical data. Adhere to safety guidelines and protocols in the laboratory environment.

Qualifications:

• Bachelor's or Master's degree in biochemistry or related discipline with 2+ years of industrial or academic experience in bioconjugation.
• Hands on experience with conjugation, purification and analytical characterization of antibody drug conjugates.
• Ability to work both independently & collaboratively and willingness to learn new bioconjugation chemistries/techniques.
• Strong communication skills, collaborative, self-motivated, goal-oriented and strong teamwork in a fast-paced setting.